During a press conference on Friday, Chief Health Officer Brett Sutton announced COVID-19 had been detected in the regional local government area of Lakes Entrance.
But this evening, it was confirmed COVID-19 had also been found in the Shepparton catchment which includes Shepparton, Kialla and parts of Shepparton North and Orrvale.
In the past month in regional Victoria, positive readings for COVID-19 wastewater fragments have been detected in Bacchus Marsh, Benalla, Bendigo, Black Rock, Healesville, Koorlong, Somers and Wangaratta.
No known cases in Benalla, Healesville or Wangaratta have been found.
Anyone who lives, works in or has visited Shepparton or Lakes Entrance from August 9 to 11 is urged to watch for the "slightest symptoms" of COVID-19 and get tested if any symptoms arise.
Health authorities said the detections were "significant" as neither Shepparton nor Lakes Entrance had any active COVID-19 cases or current exposure sites.
"People in areas with recent wastewater detections need to be especially vigilant in getting tested should they develop even the mildest of symptoms," a statement read.
There are also positive wastewater detections in metropolitan Melbourne in the Camberwell and Balwyn area and the western suburbs.Â
Victoria detected 15 new locally acquired cases of COVID-19 on Friday, with four cases still under investigation by authorities.
Eleven cases were linked to previous outbreaks, and eight had been in quarantine throughout their entire infectious period.
More to come.